Evaluation of the Borrelia burgdorferi BBA64 Protein as a Protective Immunogen in Mice
نویسندگان
چکیده
منابع مشابه
Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter.
The bba64 (P35) gene of Borrelia burgdorferi, the agent of Lyme disease, encodes a surface-exposed lipoprotein. The expression of bba64 in vitro is tightly regulated and dependent on several environmental factors. In nature, its expression is induced in the tick vector during feeding and maintained during infection of the vertebrate host. The pattern of expression of bba64 suggests that it impa...
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A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...
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Borrelia burgdorferi, the causative agent of Lyme borreliosis, is transmitted to humans from the bite of Ixodes spp. ticks. During the borrelial tick-to-mammal life cycle, B. burgdorferi must adapt to many environmental changes by regulating several genes, including bba64. Our laboratory recently demonstrated that the bba64 gene product is necessary for mouse infectivity when B. burgdorferi is ...
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Borrelia burgdorferi synthesizes an HtrA protease (BbHtrA) which is a surface-exposed, conserved protein within Lyme disease spirochetes with activity toward CheX and BmpD of Borrelia spp, as well as aggrecan, fibronectin and proteoglycans found in skin, joints and neural tissues of vertebrates. An antibody response against BbHtrA is observed in Lyme disease patients and in experimentally infec...
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To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2014
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.00824-13